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Characterization and cytocompatibility of surface modified polyamide66
Author(s) -
Shen Juan,
Li Yubao,
Zuo Yi,
Zou Qin,
Li Jidong,
Huang Di,
Wang Xiaoyan
Publication year - 2009
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31472
Subject(s) - contact angle , materials science , fourier transform infrared spectroscopy , surface roughness , membrane , wetting , gelatin , methacrylic acid , surface modification , grafting , covalent bond , chemical engineering , x ray photoelectron spectroscopy , polymer chemistry , polymer , surface finish , root mean square , polymerization , composite material , chemistry , organic chemistry , electrical engineering , engineering , biochemistry
The chemical modification of polyamide66 (PA66) membrane by graft polymerization with methacrylic acid (MAA) was initiated under ultraviolet light. Subsequently, covalent immobilization of bioactive surface was obtained by coupling gelatin to the MAA graft chains with the aid of a water‐soluble carbodiimide (WSC). The existence of carboxyl groups grafted on PA66 surface was verified quantitatively by UV–vis spectroscopy. The chemical composition, surface topography, and wettability were investigated by Fourier transform infrared (FTIR) technique, X‐ray photoelectron spectroscopy, atomic force microscopy, and water contact angle (WCA) measurement. Results showed that the WCA changed from the original value of 67.5° to the minimum value of 30° after grafting with PMAA. Original PA66 displayed a smooth surface morphology [root mean square (RMS) roughness was around 16 nm]. The modified PA66 surface exhibited an increase in roughness (RMS roughness around 21 nm). Simultaneously, the original and modified PA66 membranes were cultured with MG63 cells to investigate their cytocompatibility. The in vitro biological evaluation demonstrated that the immobilization of gelatin on PA66 membrane acted as a good template for the attachment and proliferation of cells. Also, the less toxic reagents and the moderate reaction conditions involved will be very helpful for the introduction of functional groups onto polymer surface. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009