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Mold fabrication and biological assessment of porous DNA–chitosan complexes
Author(s) -
Fukushima Tadao,
Ohno Jun,
Hayakawa Tohru,
Kawaguchi Minoru,
Inoue Yusuke,
Takeda Shoji,
Toyoda Mika,
Okahata Yoshio
Publication year - 2009
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31451
Subject(s) - chitosan , fabrication , distilled water , materials science , porosity , dna , chemical engineering , nanotechnology , chemistry , composite material , chromatography , biochemistry , medicine , alternative medicine , pathology , engineering
A previous study revealed that DNA–chitosan complex prepared from the reaction between native DNA and chitosan in aqueous solution has suitable porosity for cell seeding, is nontoxic, and causes only a mild soft‐tissue response. This simple and easy fabrication method for porous DNA–chitosan complex provides for a wide variety of applications as a scaffold material. The present study evaluated whether rinsing with PBS solution can fabricate DNA–chitosan complex in a mold and the histopathological responses of rat soft tissues to fabricated DNA–chitosan complexes. DNA–chitosan complex paste was prepared by mixing distilled water and freeze‐dried water‐rinsed DNA–chitosan complex powder. A DNA–chitosan complex disk could be fabricated by rinsing with PBS buffer and subsequently freeze‐drying the DNA–chitosan complex paste in the mold. Thus, a wide range of applications of DNA–chitosan complex for tissue engineering can be anticipated using the present easy fabrication method. The porosity of the disk was 85%, and many pores were visible in the DNA–chitosan complex (before fabrication) and in the fabricated DNA–chitosan disk. The values of the complex disks gradually reduced in the tissues although 60% of disks remained in the tissues. In conclusion, an easy fabrication method for making porous DNA–chitosan complex disks was developed. It was found that the fabrication method can delay the biodegradation of the DNA–chitosan complex disk without serious tissue responses in vivo . DNA–chitosan complex is promising as a scaffold material, and a wide range of applications of DNA–chitosan complex for tissue engineering are anticipated. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009