Premium
Comparative evaluation of different crystal‐structured calcium sulfates as bone‐filling materials
Author(s) -
Woo Kyung Mi,
Yu Bin,
Jung HongMoon,
Lee YongKeun
Publication year - 2009
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31428
Subject(s) - bone sialoprotein , osteocalcin , alkaline phosphatase , calcium , osteoblast , chemistry , runx2 , tartrate resistant acid phosphatase , osteoclast , type i collagen , acid phosphatase , biochemistry , biophysics , endocrinology , in vitro , enzyme , biology , organic chemistry
The mechanical and handling properties and biological performances of two types of calcium sulfate (βCS and αCS) as bone‐filling materials were compared. The influence of two modifiers such as hydroxypropylmethylcellose (HPMC) and fibrin was also examined. αCS showed higher strength than, and similar setting time and injectability to those of βCS. The degradation of CS in a simulated body fluid (SBF) was checked by measuring the amount of calcium released to SBF. αCS showed reduced calcium release than βCS. The modifiers tended to increase the calcium release. The MC3T3‐E1 preosteoblasts cultured on αCS showed higher levels of alkaline phosphatase (ALP) activity than those cultured on βCS. αCS strongly promoted gene expression of osteoblast phenotypes including Runx2, α1(I) collagen, osteocalcin, and bone sialoprotein. There was no significant difference in cell adhesion and proliferation between two types of CS. The addition of modifiers to CS increased cell proliferation, ALP activity, and the gene expression. The osteoclastic differentiation of RAW264.7 monocytes was checked. The cells on both types of CS produced tartrate‐resistant acid phosphatase (TRAP) activity with no significant difference. These cell response results indicated that αCS promoted osteoblast differentiation over βCS but not osteoclast differentiation. Conclusively, a particular form of commercially available αCS possesses superior properties to βCS in terms of mechanical properties and supports for osteoblast differentiation, suggesting that αCS could be an alternative to the conventionally used βCS. The addition of HPMC and fibrin could further improve the feasibility of αCS as a bone‐filling material. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009