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Construction of a multi‐functional extracellular matrix protein that increases number of N1E‐115 neuroblast cells having neurites
Author(s) -
Nakamura Makiko,
Mie Masayasu,
Mihara Hisakazu,
Nakamura Makoto,
Kobatake Eiry
Publication year - 2009
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31418
Subject(s) - neurite , laminin , fibronectin , fusion protein , extracellular matrix , microbiology and biotechnology , adhesion , cell adhesion , neuroblast , cell fusion , biophysics , chemistry , cell , biology , biochemistry , in vitro , neurogenesis , recombinant dna , gene , organic chemistry
Abstract An artificially designed fusion protein, which was designed to have strong cell adhesive activity and an active functional unit that enhances neuronal differentiation of mouse N1E‐115 neuroblast cells, was developed. In this study, a laminin‐1‐derived IKVAV sequence, which stimulates neurite outgrowth in conditions of serum deprivation, was engineered and incorporated into an elastin‐derived structural unit. The designed fusion protein also had a cell‐adhesive RGD sequence derived from fibronectin. The resultant fusion protein could adsorb efficiently onto hydrophobic culture surfaces and showed cell adhesion activity similar to laminin. N1E‐115 cells grown on the fusion protein exhibited more cells with neurites than cells grown on laminin‐1. These results indicated that the constructed protein could retain properties of incorporated functional peptides and could provide effective signal transport. The strategy of designing multi‐functional fusion proteins has the possibility for supporting current tissue engineering techniques. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009