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In vitro maturation of “biotube” vascular grafts induced by a 2‐day pulsatile flow loading
Author(s) -
Huang Haiying,
Zhou YueMin,
IshibashiUeda Hatsue,
Takamizawa Keiichi,
Ando Joji,
Kanda Keiichi,
Yaku Hitoshi,
Nakayama Yasuhide
Publication year - 2009
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31405
Subject(s) - pulsatile flow , biomedical engineering , materials science , shear stress , tissue engineering , in vivo , silicone , anatomy , chemistry , medicine , composite material , cardiology , biology , microbiology and biotechnology
Autologous vascular tissues with a small diameter, “biotubes,” were developed in vivo using a novel concept in regenerative medicine, “in‐body tissue architecture technology.” The effect of pulsatile flow in vitro was investigated on the structural and functional properties of the biotubes. Silicone rods (diameter, 3.0 mm; length, 35.0 mm), used as molds, were embedded into dorsal subcutaneous spaces of Wister rats. After 4 weeks, the autologous tubular tissues formed around the rods were harvested. Some tissues were incubated for 2 days under pulsatile flow simulating conditions in the human arteries with small caliber (wall shear stress (WSS), 15.5–77.3 dyn/cm 2 ; circumferential stress (CS), 0.6–4.5 × 10 5 dyn/cm 2 ). Upon flow loading, the sparse, randomly oriented collagen fibers in the biotubes became dense and oriented in the regular circumferential direction. Compliances (β values) of the control (ca. 30) and flow‐loaded (ca. 20) biotubes were equivalent to that of the human coronary arteries and femoral arteries, respectively. Further, upon flow loading, the burst pressure significantly increased from ca. 1000 mmHg to ca. 1800 mmHg, along with the α‐SMA‐positive cell ratio. Pulsatile flow loading in vitro for 2 days could induce biotube maturation in terms of collagen structures and mechanical properties. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009