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Biomimetic apatite deposition on polymeric microspheres treated with a calcium silicate solution
Author(s) -
Leonor Isabel B.,
Balas Francisco,
Kawashita Masakazu,
Reis Rui L.,
Kokubo Tadashi,
Nakamura Takashi
Publication year - 2009
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31395
Subject(s) - apatite , silicate , microsphere , deposition (geology) , calcium silicate , chemical engineering , calcium , materials science , mineralogy , chemistry , geology , metallurgy , composite material , sediment , paleontology , engineering
Bioactive polymeric microspheres can be prepared by means of coating them with a calcium silicate solution and subsequently soaking in a simulated body fluid (SBF). Such combination should allow for the development of bioactive microspheres for several applications in the medical field including tissue engineering carriers. Four types of polymeric microspheres, with different sizes, were used in this work: (i) ethylene‐vinyl alcohol copolymer (20–30 μm), (ii) polyamide 12 with 10% magnetite (100 μm), (iii) polyamide 12 (10–30 μm) and (iv) polyamide 12 (300 μm). These microspheres were soaked in a calcium silicate solution at 36.5°C for various periods of time under different conditions. Afterwards, they were dried in air at 60 and 100°C for 24 hr. Then, the samples were soaked in SBF for 1, 3, and 7 days. Fourier transformed infrared spectroscopy, thin‐film X‐ray diffraction, and scanning electron microscopy showed that after the calcium silicate treatment and the subsequent soaking in SBF, the microspheres successfully formed an apatite layer on their surfaces in SBF within 7 days due to the formation of silanol groups, which are effective for apatite formation. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009