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Calcium phosphate/DNA co‐precipitates encapsulated fast‐degrading polymer films for substrate‐mediated gene delivery
Author(s) -
Zhang Qiao,
Zhao Dong,
Zhang XianZheng,
Cheng SiXue,
Zhuo RenXi
Publication year - 2009
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31387
Subject(s) - calcium , gene delivery , substrate (aquarium) , polymer , phosphate , dna , chemical engineering , chemistry , materials science , gene , biochemistry , genetic enhancement , organic chemistry , biology , ecology , engineering
Calcium‐phosphate/deoxyribose nucleic acid (Ca‐P/DNA) co‐precipitates were deposited on or encapsulated in fast‐degrading polymer films with surface erosion degradation mechanism to mediate cell transfection. The polymer, cholic acid functionalized star poly( DL ‐lactide), was synthesized through the ring‐opening polymerization of DL ‐lactide initiated by cholic acid. The releases of DNA from the Ca‐P/DNA co‐precipitates deposited film and the Ca‐P/DNA co‐precipitates encapsulated film were determined and compared. The in vitro gene transfections of HEK293 cells, Hela cells, and NIH 3T3 cells showed that the expression of pGL3‐Luc plasmid could be effectively mediated by the Ca‐P/DNA co‐precipitates deposited and encapsulated polymer films. In addition, the films did not exhibit any additional cytotoxicity to the cells during the transfections, indicating that the fast‐degrading polymer films have great potential in localized gene delivery. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009