Premium
Dynamic compression modulates chondrocyte proliferation and matrix biosynthesis in chitosan/gelatin scaffolds
Author(s) -
Wang PengYuan,
Chow HsiangHong,
Lai JuinYih,
Liu HsuanLiang,
Tsai WeiBor
Publication year - 2009
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31384
Subject(s) - chondrocyte , aggrecan , extracellular matrix , gelatin , tissue engineering , compression (physics) , biomedical engineering , glycosaminoglycan , type ii collagen , scaffold , cartilage , materials science , chemistry , microbiology and biotechnology , strain (injury) , matrix (chemical analysis) , biophysics , articular cartilage , anatomy , composite material , biochemistry , biology , osteoarthritis , pathology , medicine , alternative medicine
It is well‐documented that dynamical compression stimulates biosynthesis of extracellular biomacromolecules in cartilage explant or in chondrocyte/hydrogel systems. The object of this study was to apply high‐strain dynamic compression to cell‐seeded elastic scaffolds for articular cartilage tissue engineering. Rabbit chondrocytes had been cultured in chitosan/gelatin scaffolds for 3 days before dynamic compression. The chondrocyte/scaffold constructs were subjected to short‐term (3 or 9 h) or long‐term (6 h/day for 3 weeks) cyclic compression with 40% strain and 0.1 Hz. The expression of type II collagen and aggrecan was upregulated after 3‐h of compression when compared with the free‐swelling samples. Furthermore, long‐term culture under dynamic compression facilitated cellular proliferation and deposition of glycosaminoglycan. Our results suggest that high‐strain dynamic compression combined with elastic scaffolds might benefit articular cartilage tissue engineering. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2009