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Bioactivity, pre‐osteoblastic cell responses, and osteoconductivity evaluations of the electrospun non‐woven SiO 2 –CaO gel fabrics
Author(s) -
Seol YangJo,
Kim KyoungHwa,
Kang Young Mi,
Kim In Ae,
Rhee SangHoon
Publication year - 2009
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31334
Subject(s) - tetraethyl orthosilicate , materials science , chemical engineering , electrospinning , molar ratio , simulated body fluid , spinning , sol gel , apatite , composite material , grafting , hydrolysis , polymer , chemistry , nanotechnology , organic chemistry , catalysis , scanning electron microscope , engineering
The evaluations of the fibers characteristics, bioactivity, pre‐osteoblastic cell responses, and osteoconductivity of the non‐woven SiO 2 –CaO gel fabric made by electrospinning method was carried out. Silica gels with four different calcium contents were prepared by condensation following hydrolysis of tetraethyl orthosilicate under acidic conditions. The molar ratios of Ca to Si prepared ranged from 0 to 0.15. SiO 2 –CaO gel fabrics were heat‐treated at 300°C for 3 h after spinning under an electric field of 2 kV/cm. As the Ca to Si ratio increased, the diameter of electrospun SiO 2 –CaO gel fibers increased because the viscosity of the SiO 2 –CaO gel solution increased. The apatite‐forming ability of heat‐treated, non‐woven SiO 2 –CaO gel fabric was evaluated in simulated body fluid and tended to increase with an increasing Ca to Si molar ratio. However, proliferation and differentiation tended to decrease with an increasing Ca to Si molar ratio. The sample which had the Ca to Si ratio as 0.10 showed good osteoconductivity in vivo in the calvarial defect New Zealand white rabbit model compared to that had the Ca to Si ratio as 0 and empty defect. These results strongly suggest that non‐woven SiO 2 –CaO gel fabric made by the electrospinning method has potential for application as a bone grafting material. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009