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Preparation and in vivo evaluation of apatite/collagen packed composite by alternate immersion method and Newton press
Author(s) -
Taira Masayuki,
Nezu Takashi,
Sasaki Kaori,
Saitoh Setsuo,
Kagiya Tadayoshi,
Harada Hidemitsu,
Takada Yukyo,
Araki Yoshima
Publication year - 2009
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31318
Subject(s) - materials science , apatite , composite number , sponge , glutaraldehyde , composite material , putty , immersion (mathematics) , biomedical engineering , chromatography , mineralogy , chemistry , coating , medicine , botany , mathematics , pure mathematics , biology
Further development of bio‐compatible, bio‐absorbable, and osteo‐conductive bio‐materials is desired for bone grafts in dental and medical clinics. One candidate material might be a high‐density apatite/collagen composite, which cures relatively large bone defects. To produce such a composite, we freeze‐dried type I collagen solution, cross‐linked the formed sponge by 2 wt % glutaraldehyde, immersed the insoluble sponge in CaCl 2 and Na 2 HPO 4 solutions alternately five times, and compacted the sponge by Newton press at 5000 kgf. For comparison, cross‐linked collagen without alternate immersion was also pressed. SEM/EPMA, XRD, and FTIR analyses clarified that alternate immersion successfully coated the collagen sponge with hydroxyapatite. Packed apatite/collagen composite and collagen disks 6 mm in diameter and 0.5 mm in height were implanted in the subperiostea of rabbit tibiae for 2, 4, 8, and 12 weeks to assess bio‐compatibility, bio‐absorbability, and osteo‐conductivity. Histological observations showed that the packed apatite/collagen composite was biocompatible, osteo‐conductive for up to 8 weeks, and largely bio‐absorbed at 12 weeks, while the packed collagen sponge caused an undesirable foreign body reaction, which worsened with the implantation period. The overall findings suggest that this packed apatite/collagen composite might be used as a new bio‐absorbable bone graft material. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009