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Effect of spongin derived from Hymeniacidon sinapium on bone mineralization
Author(s) -
Kim MoonMoo,
Mendis Eresha,
Rajapakse Niranjan,
Lee SangHoon,
Kim SeKwon
Publication year - 2009
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31315
Subject(s) - mineralization (soil science) , osteocalcin , chemistry , osteoblast , sponge , in vitro , fish bone , microbiology and biotechnology , alkaline phosphatase , anatomy , medicine , biochemistry , biology , enzyme , fish <actinopterygii> , botany , fishery , organic chemistry , nitrogen
Marine sponges have been known to provide a source of novel bone and cartilage replacements because of their secondary metabolites and specific skeleton structures. In particular, it has been reported that spongin as a component of fibrous skeleton, pseudokeratin, neurokeratin, horny protein, and collagen‐like protein in sponges can be used in several biomedical applications including osteoarthritis (OA). However, the pharmacological mechanism of action of spongin remains obscure. In this study, it was investigated whether spongin derived from Hymeniacidon sinapium can promote bone mineralization of osteoblast‐like MG‐63 cells. Our present study provides the first evidence that spongin is effective in activating bone mineralization. Furthermore, spongin increased ALP activity, collagen synthesis, and osteocalcin secretion in addition to bone mineralization in osteoblastic cells in vitro . In addition, it was demonstrated that spongin exerted the inhibitory effect on production of inflammatory mediators such as TNF‐α, IL‐1β, and PGE 2 in macrophage, RAW264.7 cells. These results suggest that the anti‐inflammatory effect of spongin derived from Hymeniacidon sinapium can play a critical role in bone mineralization of osteoblast‐like MG‐63 cells. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009