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A lubricious formulation exhibiting reduced thrombogenicity, cell proliferation, and protein adsorption
Author(s) -
Vicario Pasquale P.,
Lu Zichun J.,
Grigorian Irina A.,
Schottman Tom
Publication year - 2009
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31306
Subject(s) - thrombogenicity , platelet , adhesion , protein adsorption , platelet adhesiveness , biomedical engineering , materials science , adsorption , chemistry , cell adhesion , umbilical vein , immunology , in vitro , medicine , biochemistry , composite material , platelet aggregation , organic chemistry
The adhesion of human platelets, erythrocytes, and leukocytes, the adsorption of protein, and the proliferation of human umbilical vein endothelial cells (HUVEC) on the surface of electropolished stainless steel and the lumen of polyurethane tubing coated with Hydromer's lubricious Duality™ T8B formulation was evaluated. Following exposure to a platelet‐enriched suspension from citrated human whole blood, stainless steel coated with this formulation exhibited significantly reduced adhesion of platelets, erythrocytes, and granulocytes. This reduction in adhesion was confirmed using an immunohistochemical method utilizing antibodies to CD41, CD235, and CD15, respectively. The proliferation of HUVEC cells were significantly reduced when cultured on coated stainless steel. This formulation was also able to significantly reduce the adsorption of plasma proteins and the major protein in tear fluid (lysozyme) to the surface of stainless steel. The nonthrombogenic properties of Duality™ T8B after application to the lumen of polyurethane tubing were also examined. Following a short‐term (3 h) static exposure to citrated human whole blood, microscopic examination revealed that the adhesion of platelets and erythrocytes was reduced significantly, a finding confirmed using anti‐CD41 and anti‐CD235 antibodies in the immunohistochemical method. A long‐term (12 day) study yielded essentially identical results indicating a significant reduction in the adhesion of blood components on the luminal surface of coated polyurethane tubing. In summary, these data indicate that the application of Duality™ T8B onto surfaces of medical devices, such as catheters, extracorporeal circuitry, and coronary stents, could aid in reducing or preventing not only thrombus formation but also the process of restenosis. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009