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Faster and stronger vascular “Biotube” graft fabrication in vivo using a novel nicotine‐containing mold
Author(s) -
Sakai Osamu,
Kanda Keiichi,
Takamizawa Keiichi,
Sato Takayuki,
Yaku Hitoshi,
Nakayama Yasuhide
Publication year - 2009
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31300
Subject(s) - biomedical engineering , in vivo , coating , mold , fabrication , materials science , dorsum , rod , nicotine , angiogenesis , composite material , anatomy , medicine , pathology , microbiology and biotechnology , biology , alternative medicine
To accelerate the fabrication of in vivo ‐tissue engineered autologous vascular prosthetic tissues, the “Biotube,” a novel drug‐coating mold was designed. The mold was prepared by impregnating nicotine as a model drug into a gelatinous matrix coated on acrylate rods (diameter, 2 mm; length, 20 mm). Upon embedding the molds into dorsal subcutaneous pouches of rats, completely encapsulated Biotubes with significant tissue migration accompanied by rich angiogenesis and having 3.8 times as many neovessels as the uncoated controls, were formed at only 2 weeks. The wall thickness and burst strength of the Biotubes were 399.9 ± 135.2 μm and 2682.6 ± 722.6 mmHg, respectively. These values were, respectively, more than 9.6 and 3.2 times greater than the corresponding controls. Therefore, it is confidently expected that the mechanical properties of Biotubes obtained by nicotine coating make them suitable for application as vascular grafts. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009