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Development of chitosan–ellagic acid films as a local drug delivery system to induce apoptotic death of human melanoma cells
Author(s) -
Kim Sungwoo,
Liu Yongxing,
Gaber M. Waleed,
Bumgardner Joel D.,
Haggard Warren O.,
Yang Yunzhi
Publication year - 2009
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31266
Subject(s) - ellagic acid , chitosan , apoptosis , fourier transform infrared spectroscopy , materials science , programmed cell death , fibroblast , cell culture , nuclear chemistry , chemistry , biophysics , chemical engineering , biochemistry , antioxidant , biology , polyphenol , in vitro , genetics , engineering
This study was designed to develop a local chemotherapy device using chitosan as a local drug carrier and ellagic acid (EA) as an anticancer drug. We fabricated chitosan–ellagic acid (Ch–EA) films with concentrations of 0, 0.05, 0.1, 0.5, and 1% (w/v) of EA and examined the films using Fourier transform infrared spectroscopy (FTIR), X‐ray diffraction (XRD), scanning electron microscopy (SEM), and contact angle measurement. The WM115 human melanoma cell line as a skin cancer model was used to evaluate cell response to the films with the MTS assay and apoptosis assay, and HS68 human newborn fibroblast cell line as a control. With the increase in the concentration of the EA, the composite films exhibit increasing amide and ester groups and diffraction peaks of the crystallized EA and greater surface roughness and hydrophilicity. The chitosan films with 0.5 and 1% (w/v) of EA were found to have a potent antiproliferative effect on the melanoma cells by inducing apoptotic cell death. Localized effect of composites on cell behaviors has been clearly demonstrated. Our study demonstrated that the novel Ch–EA film can be potentially used in local chemotherapy. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009