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Synthesis and use of pHEMA microbeads with human EA.hy 926 endothelial cells
Author(s) -
Nyangoga Hervé,
Zecheru Teodora,
Filmon Robert,
Baslé MichelFélix,
Cincu Corneliu,
Chappard Daniel
Publication year - 2009
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31240
Subject(s) - internalization , umbilical vein , confocal microscopy , flow cytometry , stromal cell , confocal , materials science , biomedical engineering , cell culture , cancer cell , drug delivery , angiogenesis , biophysics , chemistry , cell , nanotechnology , cancer research , microbiology and biotechnology , cancer , medicine , in vitro , immunology , biochemistry , biology , geometry , genetics , mathematics
Abstract Cancer has become a major problem in public health and the resulting bone metastases a worsening factor. Facing it, different strategies have been proposed and mechanisms involved in tumor angiogenesis are being studied. Enhanced permeability retention (EPR) effect is a key step in designing new anticancer drugs. We have prepared poly 2‐hydroxyethyl methacrylate (pHEMA) microbeads to target human endothelial EA.hy 926 cells, a cell line derived from human umbilical vein endothelial cells. Microbeads were synthesized by emulsion precipitation method and carried positive or negative charges. EA.hy 926 cells were cultured in 24‐well plates and microbeads were deposited on cells at various times. Scanning and transmission electron microscopy, flow cytometry, confocal microscopy, and three‐dimensional (3D) reconstruction were used to characterize microbeads and their location outside and inside cells. Microbeads were uptaken by endothelial cells with a better internalization for negatively charged microbeads. 3D reconstruction of confocal optical sections clearly evidenced the uptake and internalization of microbeads by endothelial cells. pHEMA microbeads could represent potential drug carrier in tumor model of metastases. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009