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Residual total protein and total organic carbon levels on reprocessed gastrointestinal (GI) biopsy forceps
Author(s) -
Lappalainen Sharon K.,
Gomatam Shanti V.,
Hitchins Victoria M.
Publication year - 2009
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31202
Subject(s) - forceps , residual , materials science , total organic carbon , carbon fibers , detection limit , chromatography , biomedical engineering , chemistry , surgery , medicine , environmental chemistry , mathematics , composite material , algorithm , composite number
Many single‐use (disposable) devices are reprocessed between patient uses. In the past, a visual determination was considered the endpoint of “clean” for cleaned reusable devices. In this report, we measured quantitative endpoints by measuring residual total protein and residual total organic carbon before and after cleaning with commercially available cleaners for medical devices on 15 single‐use gastrointestinal biopsy forceps inoculated with a three‐protein test soil before and after cleaning. Each device was extracted separately and tested using Bradford's reagent for total protein and a direct digestion method for total organic carbon. Data for pre‐ and post‐cleaned devices were generated for all five cleaning cycles. The highest amounts of residual total protein and total organic carbon found on precleaned devices were 61.8 μg/cm 2 and 39.1 μg/cm 2 , respectively, on the outer surfaces. The highest amounts of residual total protein and total organic carbon on postcleaned device extracts were 4.0 μg/cm 2 and 2.2 μg/cm 2 , respectively, on the outer surfaces. All postcleaned devices were visually clean. Our results provide quantitative total protein and total organic carbon levels for cleaned single‐use biopsy forceps. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009