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In vitro cytotoxicity of maxillofacial silicone elastomers: Effect of accelerated aging
Author(s) -
Bal Bilge Turhan,
Yılmaz Handan,
Aydın Cemal,
Karakoca Seçil,
Yılmaz Şükran
Publication year - 2009
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31194
Subject(s) - silicone , cytotoxicity , elastomer , materials science , incubation , biocompatibility , mtt assay , in vitro , biomedical engineering , dentistry , composite material , medicine , chemistry , biochemistry , metallurgy
The purpose of this in vitro study was to evaluate the cytotoxicity of three maxillofacial silicone elastomers at 24, 48, and 72 h on L‐929 cells and to determine the effect of accelerated aging on the cytotoxicity of these silicone elastomers. Disc‐shaped test samples of maxillofacial silicone elastomers (Cosmesil, Episil, Multisil) were fabricated according to manufacturers' instructions under aseptic conditions. Samples were then divided into three groups: (1) not aged; (2) aged for 150 h with an accelerated weathering tester; and (3) aged for 300 h. Then the samples were placed in Dulbecco's Modified Eagle Medium/Ham's F12 (DMEM/F12) for 24, 48, and 72 h. After the incubation periods, cytotoxicity of the extracts to cultured fibroblasts (L‐929) was measured by MTT assay. The degree of cytotoxicity of each sample was determined according to the reference value represented by the cells with a control (culture without sample). Statistical significance was determined by repeated measurement ANOVA ( p < 0.01) followed by Duncan's test ( p < 0.05). All test materials in each group demonstrated high survival rates in MTT assay (Episil; 93.84%, Multisil; 88.30%, Cosmesil; 87.50%, respectively); however, in all groups, Episil material demonstrated significantly higher cell survival rate after each of the experimental incubation periods ( p < 0.05). Accelerated aging for 150 and 300 h had no significant effect on the biocompatibility of maxillofacial silicone elastomers tested ( p > 0.05). © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009