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Chitosan‐NAC nanoparticles as a vehicle for nasal absorption enhancement of insulin
Author(s) -
Wang Xin,
Zheng Chao,
Wu Zhongming,
Teng Dayong,
Zhang Xinge,
Wang Zhen,
Li Chaoxing
Publication year - 2009
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31161
Subject(s) - chitosan , nasal administration , nanoparticle , insulin , absorption (acoustics) , nuclear chemistry , zeta potential , chemistry , mucoadhesion , materials science , nanotechnology , drug carrier , pharmacology , organic chemistry , drug delivery , medicine , composite material
The purpose of this work was to investigate chitosan‐ N ‐acetyl‐ L ‐cysteine (chitosan‐NAC) nanoparticles as a potential carrier system for the nasal delivery of insulin. For the study, we used insulin‐loaded chitosan‐NAC nanoparticles (140–210 nm in diameter) prepared by in situ gelation with tripolyphosphate (TPP), with positive zeta potential values of +19.5–31.7 mV and insulin loading capacities of 13–42%. The physicochemical properties of the nanoparticles were affected by the number of thiol groups present. Mucoadhesive properties, which were evaluated by measuring the in vitro absorbed mass of mucin, of chitosan‐NAC nanoparticles were >1.8‐fold that of unmodified chitosan nanoparticles. In aqueous solution, chitosan‐NAC nanoparticles exhibited fast swelling behavior. Insulin was released from chitosan‐NAC nanoparticles in vitro in an initial burst followed by slow release. Intranasal administration of chitosan‐NAC nanoparticles in rats enhanced the absorption of insulin by the nasal mucosa compared with unmodified chitosan nanoparticles and control insulin solution. In light of these observations, the novel thiolated chitosan nanoparticles represent a promising vehicle for nasal insulin administration. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009