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Intracerebral microinjection of stannous 2‐ethylhexanoate affects dopamine turnover in cerebral cortex and locomotor activity in rats
Author(s) -
Yamada Takashi,
Jung DukYoung,
Sawada Rumi,
Tsuchiya Toshie
Publication year - 2008
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31115
Subject(s) - dopamine , microinjection , neurotoxicity , chemistry , neurotransmission , in vitro , medicine , central nervous system , cerebral cortex , endocrinology , ventricle , biochemistry , toxicity , receptor
Stannous 2‐ethylhexanoate [Sn(Oct) 2 ] is used as a catalyst for production of poly‐ L ‐lactic acid and copolymers that are implanted in cranial surgery, but reports on its effects on the central nervous system are few. We examined the effects of Sn(Oct) 2 on cell viability in vitro and on neurotransmission and behavior in the rat. Treatment of normal human astrocytes with 10 mg/mL Sn(Oct) 2 reduced mitochondrial activity to 16% of the control. Injection of Sn(Oct) 2 at 6.28 mg/kg BW (2 mg/kg BW Sn) into right lateral ventricle of the rat brain tended to increase the ambulation distance after 30 days when compared with the control group. The turnover of dopamine neurotransmission was increased in the cerebral cortex. These results suggest that Sn(Oct) 2 is cytotoxic to astrocytes in vitro . Injection of Sn(Oct) 2 into the brain had no or very weak immediate neurotoxicity, but long‐term exposure to Sn(Oct) 2 increased dopamine neurotransmission turnover. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008