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Bacterial transmission from lens storage cases to contact lenses—Effects of lens care solutions and silver impregnation of cases
Author(s) -
Vermeltfoort Pit B. J.,
Hooymans Johanna M. M.,
Busscher Henk J.,
van der Mei Henny C.
Publication year - 2008
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31102
Subject(s) - biofilm , lens (geology) , contact lens , bacteria , pseudomonas aeruginosa , materials science , staphylococcus aureus , microbiology and biotechnology , transmission (telecommunications) , biology , optics , paleontology , genetics , physics , electrical engineering , engineering
The killing efficacies of multipurpose lens care solutions on planktonic and biofilm bacteria grown in polypropylene contact lens storage cases with and without silver impregnation and effects on bacterial transmission from storage cases to silicone hydrogel contact lenses were investigated. For transmission studies, biofilms of Staphylococcus aureus 835 or Pseudomonas aeruginosa no. 3 were grown on lens storage cases and incubated with a contact lens in different multipurpose lens care solutions (Opti‐Free®Express®, ReNu® MultiPlus®, and SoloCare Aqua™) or 0.9% NaCl. In addition, planktonic bacteria were directly suspended in multipurpose solutions and their killing efficacies were determined. The numbers of transmitted live and dead bacteria on the lenses were measured using a combination of plate counting and fluorescence microscopy. The highest killing efficacies were shown by Opti‐Free® Express® for planktonic as well as for biofilm bacteria. Silver impregnation of lens cases in combination with the prescribed solution increased the killing efficacy for P. aeruginosa in biofilms, whereas effects for S. aureus were minor. Lowest numbers of live and dead bacteria were transmitted to a lens in Opti‐Free® Express® multipurpose solution, with no significant differences between lens types and no effects of silver impregnation. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2008