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Expansion and characterization of adipose tissue‐derived stromal cells cultured with low serum medium
Author(s) -
Hattori Hidemi,
Nogami Yashiro,
Tanaka Tomohiro,
Amano Yoshiko,
Fukuda Koichi,
Kishimoto Satoko,
Kanatani Yasuhiro,
Nakamura Shingo,
Takase Bonpei,
Ishihara Masayuki
Publication year - 2008
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31101
Subject(s) - adipose tissue , fetal bovine serum , stromal cell , adipogenesis , microbiology and biotechnology , mesenchymal stem cell , biology , transplantation , immunology , cell , chemistry , medicine , cancer research , endocrinology , biochemistry
Adipose tissue contains a population of cells that have extensive self‐renewal capacity and the ability to differentiate along multiple lineages. In addition, adipose tissue‐derived stromal cells (ATSCs) are able to differentiate into various cell types that may be useful for autologous cell transplantation for defects of bone, cartilage, adipose, and tendon, etc. Most protocols for in vitro cultures of ATSCs include fetal bovine serum (FBS) as a nutritional supplement. However, in some cell cultures, it involves multiple doses of FBS, which raises a concern over possible infections as well as immunological reactions that are caused by medium‐derived FBS proteins, sialic acid, etc. In this study, we were able to expand mouse ATSCs using low mouse serum media containing collagen type I, heparin‐carrying polystyrene, and fibroblast growth factor (FGF)‐2. These expanded mouse ATSCs maintained their multilineage potential for differentiation into adipocytes, osteoblasts, and chondrocytes. Therefore, this method, which uses autologous cells and low serum media, may be able to be utilized for clinical cell therapies. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008