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Antimicrobial properties and elution kinetics of linezolid‐loaded hip spacers in vitro
Author(s) -
Anagnostakos Konstantinos,
Kelm Jens,
Grün Sandra,
Schmitt Eduard,
Jung Wolfgang,
Swoboda Stefanie
Publication year - 2008
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31088
Subject(s) - linezolid , gentamicin , bone cement , antimicrobial , elution , antibiotics , staphylococcus aureus , minimum inhibitory concentration , chemistry , microbiology and biotechnology , materials science , nuclear chemistry , cement , vancomycin , chromatography , bacteria , biology , composite material , genetics
Abstract Bacterial adhesion to and ‐persistence on antibiotic‐loaded bone cement is an increasing problem. New antibiotics with good antimicrobial and pharmacokinetic properties (e.g. linezolid) may be the solution to this problem; however, few data concerning linezolid‐loaded acrylic cement are currently available. Ten gentamicin‐linezolid‐loaded hip spacers (1 g gentamicin/2.4 g linezolid/80 g PMMA; five spacers including a metallic endoskeleton, five with no metallic components) were tested in vitro against a strain of methicillin‐resistant Staphylococcus aureus with regard to antibiotic release and bacteria growth inhibition. Daily, the antibiotic elution was determined by high liquid performance chromatography (linezolid) and fluorescence polarization immunoassay (gentamicin), the bacteria growth inhibition photometrically at 546 nm. All spacers demonstrated growth inhibition for 8 days. Peak average concentrations were reached for both agents on day 1 (gentamicin−35.10 μg/mL [24.10–52.52], linezolid−36.28 μg/mL [22.87–71.76]). After 8 days, 0.97% [0.93–1.05%] of the initial amount of linezolid and 3.13% [2.85–3.31%] of gentamicin were meanly released from spacers containing a metallic endoskeleton. In those containing of simple cement these values were 1.22% [0.91–1.59%] and 2.67% [2.12–2.73%], respectively. Linezolid demonstrated acceptable elution kinetics from bone cement; however, further experimental research and animal studies should clarify any possible side effect of linezolid‐loaded cement media before definitive use in the clinical practice. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008