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Oligo‐microarray based on oligonucleotide immobilization on glass surface modified with activated acrylic acid‐ co ‐acrylamide copolymer
Author(s) -
Wu Qinghua,
Ma Wenli,
Zhu Lina,
Guo Qiuye,
Zhang Bao,
Kulageri Shivappa M.,
Zheng Wenling
Publication year - 2008
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.31069
Subject(s) - materials science , copolymer , oligonucleotide , acrylamide , acrylic acid , covalent bond , surface modification , carbodiimide , fabrication , polymer chemistry , chemical engineering , polymer , composite material , organic chemistry , chemistry , biochemistry , gene , engineering , medicine , alternative medicine , pathology
Oligo‐microarray can be fabricated on modified surface of glass slides involving immobilization of oligonucleotides. In this study, glass slides were modified with acrylic acid‐ co ‐acrylamide copolymer and EDC (1‐ethyl‐3(3‐dimethylaminopropyl)‐carbodiimide)/NHS ( N ‐hydroxysuccinimide) by covalent linkage method, and covalent immobilization of unmodified oligonucleotide on the glass surface was achieved. The platform with binding of stable and sensitive oligonucleotides was prepared for oligo‐microarray fabrication. An optimum concentration of 0.5 g/L was used for spotting. The lengths of 26 to 70 mer oligonucleotides were immobilized on the surface efficiently. The spots were ∼160 nm in diameter and their mean value of fluorescent intensity was measured in the range of 3.2 × 10 4 to 7.3 × 10 4 after hybridization. The modified glass surface was scanned by SEM and the dendritic structure was observed. Results showed that the process of preparation of the modified glass surface was simple and cost effective, and the modified surface can be used for the oligo‐microarray fabrication and also attachment of protein, PNA etc. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008