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Genetic effect of anatase on osteoblast‐like cells
Author(s) -
Sollazzo Vincenzo,
Palmieri Annalisa,
Pezzetti Furio,
Scarano Antonio,
Martinelli Marcella,
Scapoli Luca,
Massari Leo,
Brunelli Giorgio,
Caramelli Elisabetta,
Carinci Francesco
Publication year - 2008
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30912
Subject(s) - anatase , osteoblast , microbiology and biotechnology , materials science , titanium , signal transduction , osseointegration , biophysics , chemistry , biology , biochemistry , in vitro , medicine , surgery , photocatalysis , implant , metallurgy , catalysis
Titanium is the gold standard among materials used for prosthetic devices, because of its good mechanical and chemical properties. When exposed to oxygen, titanium becomes an oxide that is biocompatible and able to induce osseointegration. Three allotropic forms of titanium dioxide exist, that is brookite, rutile, and anatase. Anatase can be prepared as a colloidal suspension and then used to coat surfaces. Anatase coating (AC) can potentially have specific biological effects. Here we are testing the effect of AC on osteoblast‐like cells (MG63) by using microarray techniques to identify genes that are differently regulated in osteoblasts exposed to AC. By using DNA microarrays containing 20,000 genes, we identified in osteoblast‐like cell lines (MG‐63) cultured on AC, several genes whose expression was significantly up‐ or downregulated. They cover a broad range of functional activities: signaling transduction, immunity, cell cycle regulation, lysosomes composition and vesicular transport, cell adhesion, cytoskeleton and extracellular matrix components, proliferation, and apoptosis. The data reported constitute, to our knowledge, the first genetic portrait of AC effects. They can be relevant to a better understanding of the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008