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Mouse femoral intramedullary injection model: Technique and microCT scan validation
Author(s) -
Zilber Sébastien,
Epstein Noah J.,
Lee SheenWoo,
Larsen Meegan,
Ma Ting,
Smith Robert L.,
Biswal Sandip,
Goodman Stuart B.
Publication year - 2008
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30872
Subject(s) - intramedullary rod , arthrotomy , medicine , biomedical engineering , biomechanics , animal model , surgery , nuclear medicine , anatomy , arthroscopy , endocrinology
Abstract The murine femoral intramedullary injection model is frequently used to examine the in vivo effects of biomaterials or cancer cells. The surgical technique includes a knee arthrotomy with patellar dislocation for intramedullary access. This study examined a less invasive surgical approach of direct injection of particles via the transpatellar tendon without patellar dislocation. By using polymethylmethacrylate injection and μCT scan, we found that, compared with the traditional technique, this new approach was more reproducible, less time consuming, and achieved identical volumes of intramedullary injections. Animal morbidity and the biomechanics of the joints were also improved as a result of the simplified procedure. Furthermore, our study suggested that an intramedullary volume in excess of 10 μL can lead to major vascular filling and so should be avoided. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008