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Effect of serum metabolites of Pueraria lobata in rats on peripheral nerve regeneration: In vitro and in vivo studies
Author(s) -
Chen HsienTung,
Yao ChunHsu,
Chao PeiDawn Lee,
Hou YuChi,
Chiang HsiuMei,
Hsieh ChangChi,
Ke CherngJyh,
Chen YuehSheng
Publication year - 2008
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30868
Subject(s) - in vivo , pueraria , neurite , nerve growth factor , sciatic nerve , regeneration (biology) , axon , in vitro , chemistry , synapsin i , endocrinology , biology , medicine , anatomy , pathology , microbiology and biotechnology , receptor , biochemistry , alternative medicine , vesicle , membrane , synaptic vesicle
This study provides in vitro and in vivo evaluation of rat serum metabolites of the Pueraria lobata (SMP) on peripheral nerve regeneration. In the in vitro study, we found that the SMP caused a marked enhancement of the nerve growth factor (NGF)‐mediated neurite outgrowth and the expression of synapsin I from PC12 cells. In the in vivo study, silicone rubber chambers filled with the SMP were used to bridge a 10‐mm sciatic nerve defect in rats. At the conclusion of 8 weeks, animals from the groups treated with the SMP had a relatively more mature structure with larger mean values of myelinated axon number, endoneurial area, and total nerve area when compared with those in the controls receiving the saline only. These results suggest that the serum metabolites of Pueraria lobata can be a potential nerve growth‐promoting factor. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2008