Premium
Nitric oxide‐releasing polyurethane–PEG copolymer containing the YIGSR peptide promotes endothelialization with decreased platelet adhesion
Author(s) -
Taite Lakeshia J.,
Yang Peter,
Jun HoWook,
West Jennifer L.
Publication year - 2008
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30850
Subject(s) - polyurethane , nitric oxide , adhesion , polyethylene glycol , intimal hyperplasia , cell adhesion , peg ratio , copolymer , materials science , polymer chemistry , endothelial stem cell , ethylene glycol , adhesive , peptide , platelet , chemistry , biophysics , biochemistry , in vitro , immunology , medicine , organic chemistry , polymer , nanotechnology , composite material , biology , layer (electronics) , finance , smooth muscle , economics
Thrombosis and intimal hyperplasia are the principal causes of small‐diameter vascular graft failure. To improve the long‐term patency of polyurethane vascular grafts, we have incorporated both poly(ethylene glycol) and a diazeniumdiolate nitric oxide (NO) donor into the backbone of polyurethane to improve thromboresistance. Additionally, we have incorporated the laminin‐derived cell adhesive peptide sequence YIGSR to encourage endothelial cell adhesion and migration, while NO release encourages endothelial cell proliferation. NO production by polyurethane films under physiological conditions demonstrated biphasic release, in which an initial burst of 70% of the incorporated NO was released within 2 days, followed by sustained release over 2 months. Endothelial cell proliferation in the presence of the NO‐releasing material was increased as compared to control polyurethane, and platelet adhesion to polyethylene glycol‐containing polyurethane was decreased significantly with the addition of the NO donor. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2008