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Development of an engineering autologous palatal mucosa‐like tissue for potential clinical applications
Author(s) -
Luitaud C.,
Laflamme C.,
Semlali A.,
Saidi S.,
Grenier G.,
Zakrzewski A.,
Rouabhia M.
Publication year - 2007
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30828
Subject(s) - stratum corneum , tissue engineering , oral mucosa , biomaterial , epithelium , wound healing , pathology , soft tissue , biomedical engineering , microbiology and biotechnology , medicine , biology , immunology
The goal of this study was to optimize key processes in recreating functional and viable palatal mucosa‐like tissue that would be easy to handle and would promote wound healing. Normal human gingival fibroblasts and epithelial cells and a clinically useful biomaterial, CollaTape®, were used. Structural and ultrastructural analyses showed that the gingival fibroblasts and epithelial cells adhered to the biomaterial and proliferated. Following a 6‐day culture, using 10 5 fibroblasts and 10 6 epithelial cells, a well‐organized palatal mucosa‐like tissue was engineered. The engineered epithelium displayed various layers, including a stratum corneum, and contained cytokeratin 16‐positive cells located in the supra‐basal layer. This palatal mucosa‐like engineered tissue was designed to meet a variety of surgical needs. The biodegradable collagen membrane (CollaTape) contributed to the flexibility of the engineered tissue. This engineered innovative tissue may contribute to the reconstruction of oral soft‐tissue defects secondary to trauma, congenital defects, and acquired diseases. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007