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Sol gel derived SiO 2 ‐CaO‐MgO‐P 2 O 5 bioglass system—Preparation and in vitro characterization
Author(s) -
Balamurugan A.,
Balossier G.,
Michel J.,
Kannan S.,
Benhayoune H.,
Rebelo A. H. S.,
Ferreira J. M. F.
Publication year - 2007
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30827
Subject(s) - simulated body fluid , apatite , materials science , bioactive glass , amorphous calcium phosphate , scanning electron microscope , amorphous solid , biocompatibility , chemical engineering , sol gel , calcium , energy dispersive x ray spectroscopy , sintering , mineralogy , nuclear chemistry , composite material , nanotechnology , chemistry , crystallography , metallurgy , engineering
The synthesis of SiO 2 ‐CaO‐MgO‐P 2 O 5 bioactive glass was carried out by the sol‐gel method. Sol‐gel derived bioglass material was crushed into powder to produce pellet disks by uniaxial pressing, followed by sintering at 900°C. The biocompatibility evaluation of the formed glass was assessed through in vitro cell culture experiments and immersion studies in simulated body fluid (SBF) for different time intervals while monitoring the pH changes and the concentration of calcium and magnesium in the SBF medium. Scanning electron microscopy, X‐ray energy dispersive spectroscopy, X‐ray diffraction analysis, and FT‐IR spectroscopy studies were conducted before and after contact of the material with SBF. At first, an amorphous calcium phosphate was formed; after 21 days this surface consisted of deposited crystalline spheres of apatite. The present investigation also revealed that the sol‐gel derived quaternary bioglass system has the ability to support the growth of osteoblast‐like cells in vitro and to promote osteoblast differentiation by stimulating the expression of major phenotypic markers. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2007