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Influence of recovering collagen with bioactive glass on osteoblast behavior
Author(s) -
Andrade Ângela Leão,
Valério Patrícia,
de Goes Alfredo Miranda,
de Fátima Leite Maria,
Domingues Rosana Zacarias
Publication year - 2007
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30820
Subject(s) - osteoblast , bioactive glass , viability assay , simulated body fluid , incubation , type i collagen , materials science , cell growth , chemistry , cell , biochemistry , scanning electron microscope , in vitro , composite material , biology , endocrinology
Bioactive ceramics have interesting properties from the biological standpoint, but their effects on cellular events remain partially unknown. In the current work, we investigated cellular viability, proliferation, and metabolic activity of rat primary osteoblasts in contact with four different samples: type I collagen, bioactive glass‐coated collagen (GC), and both samples submitted to immersion for 5 days in a simulated body fluid. The bioactive glass coating was obtained from a sol‐gel process. The cell viability, the alkaline phosphate, the collagen secretion, and the nitric oxide production by osteoblast were measured after 72 h of incubation in the presence of the samples. The GC that was immersed for 5 days in a simulated body fluid solution showed an increase in osteoblast viability and proliferation when it was compared with control and the other samples. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007