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Development of the wing‐attached rod for acceleration of “Biotube” vascular grafts fabrication in vivo
Author(s) -
Sakai Osamu,
Kanda Keiichi,
IshibashiUeda Hatsue,
Takamizawa Keiichi,
Ametani Akihiro,
Yaku Hitoshi,
Nakayama Yasuhide
Publication year - 2007
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30789
Subject(s) - wing , fabrication , in vivo , acceleration , vascular graft , materials science , biomedical engineering , engineering , medicine , structural engineering , biology , physics , classical mechanics , pathology , alternative medicine , microbiology and biotechnology
To accelerate the fabrication of in vivo tissue‐engineered autologous vascular prosthetic tissues, the “Biotube,” a novel wing‐attached rod mold was designed for a tissue rolling technique based on a two‐step in body tissue incubation (IBTI) process. The new mold consisted of a silicone rod (3‐mm diameter, 23‐mm length) partly connected to a poly(ethylene terephthalate) film (a wing, 23 × 19 × 0.1 mm). While the molds were embedded into the dorsal subcutaneous pouches of rabbits for 2 weeks (primary IBTI), they were encapsulated fully with thin connective tissues. After removal of the wing materials, the remaining saccular membranous tissues were rolled up on the core tubular tissues that had formed around the silicone rods. Following another 2‐week embedding of the assembled tissues (secondary IBTI), the layered tissues fused to each other to form compliant and stiff tubular tissues, “Rolled Biotubes.” The wall thickness of the Rolled Biotubes was about 800 μm and the burst strength was about 4000 mmHg, both of which were significantly higher than those of Biotubes prepared by one‐step, 4‐week IBTI or two‐step, 2‐week IBTI ( p < 0.05). A Rolled Biotube could be applied as middle or large caliber arterial prostheses. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2007