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Inhibitory effects of root canal sealers on the expression of inducible nitric oxide synthase in lipopolysaccharide‐stimulated murine macrophage cells
Author(s) -
Lee Dong Hee,
Lim BumSoon,
Lee YongKeun,
Kim Na Ryoung,
Yang HyeongCheol
Publication year - 2007
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30770
Subject(s) - lipopolysaccharide , nitric oxide synthase , root canal , macrophage , nitric oxide , western blot , chemistry , microbiology and biotechnology , inflammation , in vitro , pharmacology , biochemistry , immunology , biology , medicine , dentistry , gene , organic chemistry
Excessive production of nitric oxide (NO) is associated with inflammation. In the present study, we examined the effects of root canal sealers (N2 Universal, Sealapex, and AH26) on NO production and inducible NO synthase (iNOS) expression in lipopolysaccharide (LPS)‐stimulated RAW 264.7 macrophages. Root canal sealers decreased NO synthesis in LPS‐induced RAW 264.7 macrophages in a dose‐dependent manner. RT‐PCR and Western blot analysis demonstrated markedly lower levels of iNOS mRNA and protein in LPS‐activated macrophage cells treated with root canal sealers compared with untreated cells. From these results, we conclude that root canal sealers do not inhibit NO synthesis by direct inhibition of the enzyme, but rather through inhibition of iNOS mRNA expression (leading to a decrease in iNOS protein expression). Our data, therefore, suggest that root canal sealers may be an effective inhibitor of LPS‐induced inflammatory effects in macrophage cells. Further in vitro and in vivo studies are necessary to confirm the effects of root canal sealers on the inflammatory processes. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007