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Factors related to contraction and mechanical strength of collagen gels seeded with canine endotenon cells
Author(s) -
Chen MengYi,
Sun Yulong,
Zhao Chunfeng,
Zobitz Mark E.,
An KaiNan,
Moran Steven L.,
Amadio Peter C.
Publication year - 2007
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30757
Subject(s) - contraction (grammar) , seeding , materials science , biophysics , biomedical engineering , chemistry , biology , medicine , agronomy
Fibroblasts can construct a hydrated collagen lattice to a tissue‐like structure that is greatly influenced by initial culture conditions. The purpose of this study was to investigate the effects of cell concentration and collagen concentration on the contraction kinetics and mechanical properties of resultant endotenon‐derived fibroblast‐seeded collagen lattice. The experiment was designed to evaluate the effect of cell concentration (0, 0.25, 0.5, and 1.0 10 6 cells/mL) and collagen concentration (0.5, 1.0, 1.5, and 2.0 mg/mL). Collagen lattice contraction was recorded for 42 days, after which time the lattices were mechanically tested. The collagen lattices seeded with higher initial cell concentration had a shorter contraction lag phase ( p < 0.01), and exhibited a higher ultimate stress ( p < 0.01) and instantaneous and equilibrium modulus ( p < 0.01) than those seeded with a lower initial cell concentration. The collagen lattices cultured with a lower initial collagen concentration also had a shorter contraction lag phase, and exhibited greater instantaneous and equilibrium modulus ( p < 0.01) than those cultured with higher initial collagen concentration. The collagen lattices of initial 0.5 mg/mL collagen concentration had the highest value of ultimate stress ( p < 0.03). © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2007