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Chitosan‐phosphorylated chitosan polyelectrolyte complex hydrogel as an osteoblast carrier
Author(s) -
Li QuanLi,
Chen ZhiQing,
Darvell Brian W.,
Liu LaiKui,
Jiang HongBing,
Zen Quan,
Peng Qian,
Ou GuoMin
Publication year - 2007
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30753
Subject(s) - chitosan , polyelectrolyte , self healing hydrogels , osteoblast , chemical engineering , materials science , phosphorylation , chemistry , polymer chemistry , polymer , in vitro , biochemistry , composite material , engineering
To simulate extra‐cellular matrix, a novel three‐dimensional scaffold of polyelectrolyte complex (PEC) hydrogel as an osteoblast carrier was synthesized. First, chitosan, a natural glycosaminoglycan, was modified by phosphorylation to obtain a water‐soluble phosphorylated chitosan ( P ‐content: 10.7 mass%). The PEC hydrogel was then formed from equal volumes of 0.173 mass% phosphorylated chitosan in water and 1 mass% chitosan in 1% (V/V) acetic acid solution. Rat osteoblasts were seeded in the hydrogel. The PEC hydrogel had a three‐dimensional hierarchically‐porous structure and good cytobiocompatibility for osteoblasts in vitro . It is concluded that the PEC hydrogel is a promising material as an osteoblast carrier. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2007