Premium
Cell/surface interactions and adhesion on Ti‐6Al‐4V: Effects of surface texture
Author(s) -
Chen J.,
Mwenifumbo S.,
Langhammer C.,
McGovern J.P.,
Li M.,
Beye A.,
Soboyejo W. O.
Publication year - 2007
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30741
Subject(s) - vinculin , adhesion , materials science , cell adhesion , texture (cosmology) , context (archaeology) , actin , staining , biophysics , microbiology and biotechnology , composite material , pathology , biology , medicine , computer science , artificial intelligence , paleontology , image (mathematics)
This paper presents the results of an experimental study of the effects of surface texture on the interactions between human osteo‐sarcoma (HOS) cells and Ti‐6Al‐4V. These include the Ti‐6Al‐4V with polished (smooth); Al 2 O 3 blasted (rough); and laser micro‐grooved geometries with controlled spacings and depths. Immuno‐fluorescence staining of adhesion proteins (actin and vinculin) was used to study the spreading and adhesion of HOS cells in 2 day culture experiments. Quantitative measures of adhesion were also obtained using an enzymatic detachment assay. The results are discussed within the context of existing theories of cell adhesion. The implications of the results are also examined for the design of textured surfaces in biomedical systems. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007