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Protein adsorption and zeta potentials of a biphasic calcium phosphate ceramic under various conditions
Author(s) -
Zhu Xiangdong,
Fan Hongsong,
Li Dongxiao,
Xiao Yumei,
Zhang Xingdong
Publication year - 2007
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30706
Subject(s) - zeta potential , adsorption , protein adsorption , ionic strength , chemistry , electrophoresis , lysozyme , microelectrophoresis , phosphate , calcium , surface charge , chromatography , ceramic , aqueous solution , chemical engineering , materials science , organic chemistry , biochemistry , nanotechnology , nanoparticle , engineering
An investigation on the relationship between protein adsorption and zeta potentials of a biphasic calcium phosphate (BCP) ceramic was carried out. Zeta potentials of the BCP ceramic particles were measured at various aqueous solutions. Bovine serum album (BSA) and its competitive adsorption with lysozyme (LSZ) on BCP were investigated using conventional protein adsorption and polyacrylamide gel electrophoresis (PAGE) methods. The results showed that zeta potential and the amount of adsorbed BSA were both influenced by pH, ionic strength, Ca 2+ and PO 4 3−concentrations in the buffers. The variation tendencies of BSA adsorption were consistent with that of zeta potentials to some extent. The co‐adsorption of BSA and LSZ on BCP was confirmed by the PAGE gel pattern. The semi‐quantitative analysis for the detected protein bands proved that LSZ had higher affinity for BCP than BSA and would preferentially bind to the surface. Electrostatic interaction played an important role in protein adsorption on the surface of the BCP ceramic particles. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006