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Growing tissue‐like constructs with Hep3B/HepG2 liver cells on PHBV microspheres of different sizes
Author(s) -
Zhu Xin Hao,
Wang ChiHwa,
Tong Yen Wah
Publication year - 2007
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30698
Subject(s) - microsphere , cell culture , in vitro , scaffold , emulsion , albumin , tissue engineering , biomedical engineering , cell growth , materials science , chemistry , chemical engineering , biochemistry , biology , medicine , genetics , engineering
In this study, an oil‐in‐water emulsion solvent evaporation technique was used to fabricate poly(3‐hydroxybutyrate‐ co ‐3‐hydroxyvalerate) (PHBV, 8% PHV), microspheres as scaffold, to guide liver cell growth. Human hepatoma cell lines, HepG2 and Hep3B, were cultured in vitro on both the microspheres and polymer films. SEM and optical microscope images showed that multilayer cells were formed among the microspheres to bridge them together and developed into cell‐construct aggregates after 1 week of culture. MTT results showed that the cell proliferation on the microspheres was more than two times higher than that on the films after 12 days of culture. The cells seeded on microspheres secreted albumin 2–4 times more than that on the positive control after 1 week of culture, which indicated that this hepatic function was greatly improved by the aggregation of cells on microspheres. Although HepG2 failed to express P‐450 activity, this hepatic function was preserved when Hep3B cultured on microspheres. All the results indicated that PHBV microspheres are appropriate scaffolds for liver tissue engineering. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006