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Hydroxyapatite formed on/in agarose gel induces activation of blood coagulation and platelets aggregation
Author(s) -
Arimura Shinichiro,
Kawahara Koichi,
Biswas Kamal Krishna,
Abeyama Kazuhiro,
Tabata Masashi,
Shimoda Toru,
Ogomi Daisuke,
Matsusaki Michiya,
Kato Shinya,
Ito Takashi,
Sugihara Kazumasa,
Akashi Mitsuru,
Hashiguchi Teruto,
Maruyama Ikuro
Publication year - 2007
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30684
Subject(s) - agarose , chemistry , sepharose , coagulation , crystallinity , materials science , adsorption , chemical engineering , chromatography , biochemistry , organic chemistry , medicine , crystallography , psychiatry , enzyme , engineering
We reported earlier that hydroxyapatite (HA) formed on/in agarose gels (HA/agarose) produced by alternate soaking process is a bone‐filling material possessing osteoconductive and hemostatic effects. This process could allow us to make bone‐like apatite that was formed on/in organic polymer hydrogel matrices. Here, we investigated the mechanism of hemostasis induced by HA/agarose and found that HA/agarose, but not agarose or HA powder, significantly shortened activated partial thromboplastin time (APTT). While HA/agarose did not show significant platelet aggregation, it markedly enhanced adenosine diphosphate (ADP)‐induced platelet aggregation. Moreover, Western blot analysis revealed selective adsorption of vitronectin onto HA/agarose. We also observed marked differences between HA powder and HA/agarose in their XRD patterns. The crystallinity of HA powder was much higher compared to that of HA/agarose. Furthermore, 50–100 nm of tube‐form aggregations was observed in HA powder on the other hand 100–200 nm of particles was observed in HA/agarose by SEM observation. Thus 100–200 nm of low crystallized particles on the surface structure of HA/agarose may play an important role in hemostasis. Our results demonstrated a crucial role of HA/agarose in the mechanism of hemostasis and suggested a potential role for HA/agarose as a bone‐grafting material. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006