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Photocrosslinkable hydrogel for myocyte cell culture and injection
Author(s) -
Yeo Yoon,
Geng Wenliang,
Ito Taichi,
Kohane Daniel S.,
Burdick Jason A.,
Radisic Milica
Publication year - 2007
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30667
Subject(s) - self healing hydrogels , chitosan , ethylene glycol , peg ratio , myocyte , c2c12 , viability assay , in vivo , materials science , biomedical engineering , chemistry , tissue engineering , biophysics , cell , polymer chemistry , in vitro , biochemistry , microbiology and biotechnology , medicine , organic chemistry , biology , finance , myogenesis , economics
Conventional treatment options for myocardial infarction are limited by the inability of mature myocardium to regenerate after injury. Although functional improvements after injection of cells and growth factors have been demonstrated, the clinical utility of this procedure has been hampered by poor cell localization, low survival, and rapid clearance of injected growth factors. The main objective of this study was to evaluate the applicability of a hydrogel, based on photocrosslinkable chitosan and acryloyl‐poly(ethylene glycol)‐RGDS (Az‐chitosan/Acr‐PEG‐RGD) for myocyte cell culture and myocardial injection. Chitosan was modified with photoreactive azidobenzoic acid and Acr‐PEG‐RGD was synthesized by reacting YRGDS with an equimolar amount of acryloyl‐PEG‐ N ‐hydroxysuccinimide. For injection and encapsulation each polymer was dissolved in Di‐H 2 O (pH 6.4), the solutions were mixed and crosslinked by UV application (4 mW/cm 2 ). C2C12 myoblasts proliferated and differentiated on hydrogels containing 5 m M RGD but not on the pure photocrosslinked chitosan. In vitro , the crosslinked hydrogels retained 80% of encapsulated VEGF for 24 days. Live/dead staining of neonatal rat cardiomyocytes encapsulated into Az‐chitosan/Acr‐PEG‐RGD hydrogels indicated high cell viability upon UV crosslinking. Ex vivo , we localized the hydrogel on the surface and in the ventricle wall of an adult rat heart by brief (2 min) UV light application. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006