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Polypeptide multilayer nanofilm artificial red blood cells
Author(s) -
Palath Naveen,
Bhad Sujaykumar,
Montazeri Reza,
Guidry Christopher A.,
Haynie Donald T.
Publication year - 2007
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30661
Subject(s) - ethylenediaminetetraacetic acid , plga , hemoglobin , materials science , fluorescence , absorbance , lysine , biophysics , chemistry , chemical engineering , amino acid , nanotechnology , nanoparticle , chromatography , biochemistry , chelation , organic chemistry , physics , quantum mechanics , biology , engineering
Reliable encapsulation of hemoglobin (Hb) within polypeptide multilayer nanofilms has been achieved by a template‐based approach, and protein functionality has been demonstrated postencapsulation. The method is general in scope and could be useful for many other encapsulants. Met‐Hb was adsorbed onto 5 μm‐diameter CaCO 3 microparticles, and the Hb‐coated particles were encapsulated within a multilayer nanofilm of poly( L ‐glutamic acid) (PLGA) and poly( L ‐lysine) (PLL) by layer‐by‐layer assembly. The CaCO 3 templates were then dissolved within the PLGA/PLL nanofilms by addition of ethylenediaminetetraacetic acid. Encapsulation of Hb was proved by fluorescence microscopy, the pH‐dependence of retention of Hb was determined by visible wavelength absorbance, and conversion of the encapsulated met‐Hb to deoxy‐Hb and oxy‐Hb was demonstrated by spectroscopic analysis of the Soret absorption peak under various conditions. It thus has been shown that control of Hb oxygenation within polypeptide multilayer nanofilm artificial cells is possible, and that Hb thus encapsulated can bind, release, and subsequently rebind molecular oxygen. This work therefore represents an advance in the development of polypeptide multilayer film artificial red blood cells. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006