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Microcontact printing of laminin on oxygen plasma activated substrates for the alignment and growth of Schwann cells
Author(s) -
Wang DeYao,
Huang YiCheng,
Chiang Hongsen,
Wo Andrew M.,
Huang YiYou
Publication year - 2007
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30616
Subject(s) - microcontact printing , materials science , polydimethylsiloxane , extracellular matrix , laminin , scaffold , cell growth , schwann cell , regeneration (biology) , biophysics , microbiology and biotechnology , biomedical engineering , nanotechnology , chemistry , biology , biochemistry , medicine
Abstract Microenvironment mimicking biological situation is a vital issue in tissue regeneration. With much progress being made, one of the major challenges remains to develop a convenient method to fabricate the scaffold microenvironment suitable for cell attachment and proliferation. This article demonstrates the efficacy of microcontact printed laminin, an extracellular matrix protein, on three different oxygen plasma treatment substrates—tissue culture polystyrene, poly(methyl methacrylate) films, and chitosan films—for alignment and growth of the Schwann cells in in vitro culturing. Replica molding of polydimethylsiloxane elastomeric stamps, fabricated from patterned SU‐8 structure on silicon master, was used to print laminin on the three substrates. Pattern and growth of Schwann cells for low (10 3 cells/cm 2 ) and increased cell density (2 × 10 4 cells/cm 2 ) on the varied substrates with and without microcontact printed laminin were characterized. Results of in vitro cell culture of Schwann cells showed a high degree of cell orientation on the laminin‐micropatterned substrates for both cell densities. However, different cell seeding densities will strongly impact the morphology and orientation of Schwann cells. Microcontact printing proves to be a convenient means to pattern cell‐recognition molecules on scaffold for cell‐guilded growth in tissue regeneration. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007