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Effect of storage upon material properties of lyophilized porcine extracellular matrix derived from the urinary bladder
Author(s) -
Freytes Donald O.,
Tullius Robert S.,
Badylak Stephen F.
Publication year - 2006
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30491
Subject(s) - extracellular matrix , biomedical engineering , sterilization (economics) , elongation , materials science , chemistry , ultimate tensile strength , composite material , medicine , biochemistry , monetary economics , economics , foreign exchange market , foreign exchange
Xenogeneic extracellular matrices (ECMs) have been developed as off‐the‐shelf biologic scaffolds that have been effectively used in preclinical and clinical applications for tissue reconstruction. Such materials must be suitable for terminal sterilization and capable of storage for extended periods of time without significant changes in material properties and bioactivity. Material properties of interest for ECM scaffolds include hydrostatic permeability index (PI), uniaxial maximum load and elongation, maximum tangential stiffness (MTS), suture retention strength (SRS), and ball‐burst strength (BBS). The present study evaluated these material properties for lyophilized forms of an ECM scaffold derived from the porcine urinary bladder, termed urinary bladder matrix (UBM), that was terminally sterilized by e‐beam irradiation at 22 kGy and stored at room temperature (RT; 20–24°C) or refrigerated temperature (REFT; 4–8°C) for up to 12 months. UBM devices showed no change in SRS, BBS, and hydrostatic PI after the evaluation period. Lyophilized devices stored at RT showed an increase in maximum load and MTS while devices stored at REFT showed an increase in maximum elongation after 1 year of storage ( p < 0.05). These results indicate that structural changes in the UBM device may slowly occur as a function of prolonged storage and storage temperature. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006