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TGF‐β1 immobilized tri‐co‐polymer for articular cartilage tissue engineering
Author(s) -
Chou ChengHung,
Cheng Winston T. K.,
Lin ChienCheng,
Chang ChihHung,
Tsai ChienChen,
Lin FengHuei
Publication year - 2006
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30432
Subject(s) - cartilage , chemistry , tissue engineering , extracellular matrix , chondroitin sulfate , scaffold , hyaluronic acid , type ii collagen , carbodiimide , matrix (chemical analysis) , chondrogenesis , sponge , in vitro , biophysics , biomedical engineering , anatomy , biochemistry , glycosaminoglycan , biology , chromatography , medicine , botany
Tri‐co‐polymer with composition of gelatin, hyaluronic acid and chondroitin‐6‐sulfate has been used to mimic the cartilage extracellular matrix as scaffold for cartilage tissue engineering. In this study, we try to immobilize TGF‐β1 onto the surface of the tri‐co‐polymer sponge to suppress the undesired differentiation during the cartilage growth in vitro . The scaffold was synthesized with a pore size in a range of 300–500 μm. TGF‐β1 was immobilized on the surface of the tri‐co‐polymer scaffold with 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimid (EDC) as a crosslinking agent. Tri‐co‐polymer scaffolds with and without TGF‐β1 were seeded with porcine chondrocytes and cultured in a spinner flask for 2, 4, and 6 weeks. The chondrocytes were characterized by the methods of immunohistochemical staining with anti‐type II collagen and anti‐S‐100 protein monoclonal antibody, and RT‐PCR. After culturing for 4 weeks, chondrocytes showed positive in S‐100 protein, Alcian blue, and type II collagen for the scaffold with TGF‐β1 immobilization. There is no observed type I and type X collagen expression in the scaffolds from the observation of RT‐PCR. In addition, the scaffold without TGF‐β1 immobilization, type X collagen, can be detected after cultured for 2 weeks. Type I collagen was progressively expressed after 4 weeks. These results can conclude that TGF‐β1 immobilized scaffold can suppress chondrocytes toward prehypertrophic chondrocytes and osteolineage cells. The tri‐co‐polymer sponge with TGF‐β1 immobilization should have a great potential in cartilage tissue engineering in the future. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006