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Titanium implant osseointegration with calcium pyrophosphate in rabbits
Author(s) -
Koo Samuel,
König Bruno,
Allegrini Sérgio,
Yoshimoto Marcelo,
Carbonari Marcelo José,
MitriLuiz Fábio Franceschini
Publication year - 2006
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30383
Subject(s) - calcium , alizarin red , pyrophosphate , osseointegration , materials science , calcein , calcification , calcium pyrophosphate , in vivo , cytotoxicity , crystallinity , serial dilution , nuclear chemistry , resorption , biomedical engineering , staining , implant , chemistry , in vitro , pathology , biochemistry , biology , surgery , medicine , composite material , alternative medicine , microbiology and biotechnology , membrane , metallurgy , enzyme
The objective of this study was to characterize calcium pyrophosphate material, evaluate its in vitro cytotoxicity, and assess its ability to induce bone formation. X‐ray diffraction (XRD) was used to deterimne crystallinity and phases present in material. Serial dilutions of extracts, from 10‐day dissolution tests in modified Eagle's medium, were exposed for 24 h to mouse fibroblasts and cytotoxicity assessed via viable staining. In vivo performance was determined by placing Ti screws with and without calcium pyrophosphate agglutinated with marrow adipose tissue in the tibiae of eight rabbits. New bone formation around test and control implants was evaluated histomorphometrically by using three fluorochrome labels: alizarin, calcein, and tetracycline. After 8 postoperative weeks, the animals were killed and specimens were retrieved and processed for fluorescence and light microscopic analysis. Calcium pyrophosphate showed no cytotoxicity and the XRD showed that the main phase of the analyzed sample corresponded to β‐calcium pyrophosphate. The largest fluorochrome labeling area occurred during the fourth and fifth postoperative weeks, in both control and experimental groups. Histologically, the bone neoformation occurred in regions where the calcium pyrophosphate was resorbed. The morphometric analysis showed implants placed with calcium pyrophosphate resulted in smaller polyfluorochrome labeling area ( p < 0.05). © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006