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Quartz‐crystal microbalance‐dissipation technique for the study of initial adsorption of fibronectin onto tresyl chloride‐activated titanium
Author(s) -
Hayakawa T.,
Yoshinari M.,
Nemoto K.
Publication year - 2005
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30207
Subject(s) - quartz crystal microbalance , titanium , fibronectin , materials science , adsorption , chloride , dissipation , chemical engineering , chemistry , metallurgy , organic chemistry , biochemistry , extracellular matrix , engineering , physics , thermodynamics
The immobilization of cell‐adhesive proteins onto titanium implants improves biological response at the implant–tissue interface. Previous studies demonstrated the easy and direct attachment of fibronectin onto titanium with the use of a 2,2,2‐trifluoroethanesulfonyl chloride (tresyl chloride) activation technique. The present study investigated the initial adsorption behavior of fibronectin on tresyl chloride‐activated titanium by the quartz‐crystal microbalance‐dissipation (QCM‐D) technique. The crystal resonant frequency and the dissipation shift of the oscillator were simultaneously measured by the injection of fibronectin/phosphate‐buffered saline solution (pH = 7.4). The tresyl chloride‐activated titanium surface showed a faster and greater decrease in frequency than that of untreated titanium, indicating that a greater amount of fibronectin was adsorbed in the former case during a 120‐min adsorption. The dissipation‐frequency plots revealed that, during the initial stage of adsorption, the bond between fibronectin and tresyl chloride‐activated titanium is stronger than that between fibronectin and untreated titanium. The QCM‐D technique can provide new insights into the adsorption mechanism of fibronectin. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater