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Preparation and evaluation of poly( L ‐lactide‐ co ‐glycolide) (PLGA) microbubbles as a contrast agent for myocardial contrast echocardiography
Author(s) -
Cui Wenjin,
Bei Jianzhong,
Wang Shenguo,
Zhi Guang,
Zhao Yuying,
Zhou Xiaoshu,
Zhang Hanwei,
Xu Yong
Publication year - 2005
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30189
Subject(s) - microbubbles , plga , mechanical index , biomedical engineering , materials science , ventricle , contrast (vision) , emulsion , ultrasound , chemistry , medicine , nanotechnology , nanoparticle , radiology , cardiology , artificial intelligence , computer science , biochemistry
A kind of absorbable PLGA microbubble‐based contrast agent (PLGA microspheres with porous or hollow inner structure) was fabricated by an improved double emulsion‐solvent evaporation method. The contrast efficiency was evaluated and proved both in vitro and in vivo . By adjusting the polymer concentration and volume of the inner aqueous phase during the fabrication of microbubbles, the inner structure of the microbubbles could be controlled. Both air‐filled and perfluoropropane‐filled microbubbles can opacify the left ventricle. However, when compared with air‐filled microbubbles, perfluoropropane‐filled microbubbles can produce significantly longer enhancement in left ventricle in the dog model due to the lower diffusivity and lower solubility of perfluoropropane in blood. A suspension of perfluoropropane‐filled PLGA microbubbles (1.8 μm average microbubbles size, 2 × 10 8 microbubbles/mL concentration) has successfully and safely achieved myocardial opacification in closed‐chest dogs. A perfusion defect was observed in both of the two dogs with acute myocardial infarction with Power Contrast Imaging (PCI) triggered technology. In the examination of contrast in both ventricular and myocardial opacification, the high mechanical index (MI) was found to have superior contrast sensitivity over the low MI for PLGA‐based contrast agents. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 73B: 171–178, 2005