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Inflammatory cytokines reaction elicited by root‐end filling materials
Author(s) -
Huang Tsui Hsien,
Yang Chi Chiang,
Ding Sinn Jyh.,
Yeng Ming.,
Kao Chia Tze,
Chou Ming Yung
Publication year - 2005
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30182
Subject(s) - mineral trioxide aggregate , dentistry , proinflammatory cytokine , bone tissue , viability assay , materials science , inflammation , chemistry , microbiology and biotechnology , cell , andrology , biomedical engineering , immunology , medicine , biochemistry , biology
Cytokines are inflammation in the list of tissue reactions that cytokines control of cell and tissue growth, development, and differentiation. Root‐end filling materials often contact with existing periapical tissue, and they need to be biocompatible with remnant periapical tissue. The aim of this study was to focus the effects of the root end filling materials on bone cell viability and expression of inflammatory cytokines and their role in maintaining health and stability of the restored dental tissues. Calcium hydroxide‐based (Life), zinc oxide eugenol‐based (Super EBA), and mineral trioxide aggregate‐based (MTA) root‐end filling materials were used to investigate their effect on a human osteosarcoma cell line (U2OS). The cell attachment assay was observed microscopically, and the expression of interleukin‐2, ‐4, and ‐10 were quantified by enzyme‐linked immunosorbent assay. Any resultant difference between the root‐end filling material was analyzed statistically by one‐way analysis of variance. The results showed that the best cell attachment to root‐end filling material occurred with MTA. The IL‐4 (0.824 ± 0.396) and IL‐10 (2.06 ± 1.24) levels were greater for the MTA group, whereas IL‐2 expression for the three kinds of root‐end filling materials was similar. All materials were able to induce expression of inflammatory cytokines from cultured bone cells. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 73B: 123–128, 2005