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Characterization of chemisorbed hyaluronic acid directly immobilized on solid substrates
Author(s) -
Suh Kahp Y.,
Yang Jen Ming,
Khademhosseini Ali,
Berry David,
Tran ThanhNga T.,
Park Hyongshin,
Langer Robert
Publication year - 2004
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30152
Subject(s) - chemistry , alginic acid , hyaluronic acid , silanol , polymer chemistry , biopolymer , silicon dioxide , chondroitin sulfate , sulfate , chemical engineering , hydrogen bond , carboxylic acid , glucuronic acid , polymer , organic chemistry , polysaccharide , molecule , glycosaminoglycan , biochemistry , genetics , engineering , biology , catalysis
Hyaluronic acid (HA) has a number of potential biomedical applications in drug delivery and tissue engineering. For these applications, a prerequisite is to understand the characteristic of HA films directly immobilized to solid substrates. Here, we demonstrate that high molecular weight HA can be directly immobilized onto hydrophilic substrates without any chemical manipulation, allowing for the formation of an ultrathin chemisorbed layer. Hyaluronic acid is stabilized on these surfaces through hydrogen bonding between the hydrophilic moieties in HA [such as carboxylic acid (COOH) or hydroxyl (OH) groups] with silanol (SiOH), carboxylic acid or hydroxyl groups on the hydrophilic substrates. Despite the water solubility, the chemisorbed HA layer remained stable on glass or silicon oxide substrates for at least 7 days in phosphate‐buffered saline. Furthermore, HA immobilized on silicon and other dioxide surfaces in much higher quantities than other polysaccharides including dextran sulfate, heparin, heparin sulfate, chondroitin sulfate, dermatan sulfate, and alginic acid. This behavior is related to the molecular entanglement and intrinsic stiffness of HA as a result of strong internal and external hydrogen bonding as well as high molecular weight. These results demonstrate that HA can be used to coat surfaces through direct immobilization. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 72B: 292–298, 2005