Premium
Biocompatible oligochitosans as cationic modifiers of alginate/Ca microcapsules
Author(s) -
Orive G.,
Bartkowiak A.,
Lisiecki S.,
De Castro M.,
Hernández R. M.,
Gascòn A. R.,
Pedraz J. L.
Publication year - 2005
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30146
Subject(s) - depolymerization , biocompatible material , chitosan , molar mass , cationic polymerization , polysaccharide , membrane , chemistry , chemical engineering , capsule , molar ratio , chromatography , materials science , polymer chemistry , polymer , biomedical engineering , biochemistry , organic chemistry , catalysis , medicine , botany , biology , engineering
In the past, it has been proven that by properly adjusting the molecular mass of the oligochitosan samples, it is possible to optimize the formation of rigid, biocompatible capsules with semipermeable membranes under physiological conditions. In this study, the feasibility of four oligochitosan samples, with varying molar masses ( M n in range 3–5 kDa), as biocompatible coatings of alginate/Ca capsules was investigated. By selection of appropriate depolymerization and purification methods we obtained oligochitosan samples that appeared to be noncytotoxic for C 2 C 12 myoblasts and did not influence the mammalian cell metabolism especially in relative short time during the process of capsule formation. Furthermore, oligochitosans can be used as a tool to reduce the membrane cut‐off of the alginate capsules. However, such reduction, as well as mechanical resistance of formed microcapsules, depend on MM of the cationic polysaccharide and the chemical composition of the alginate (mannuronic/guluronic acid ratio). Here, we address that the use of low molar mass chitosan (< 5000 g/mol) permits the formation of mechanical stable capsules at physiological pH, which represents a strong advantage over other chitosan‐based chemistries. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2005