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Enzymatic stabilization of gelatin‐based scaffolds
Author(s) -
Broderick Emmett P.,
O'Halloran Damien M.,
Rochev Yury A.,
Griffin Martin,
Collighan Russell J.,
Pandit Abhay S.
Publication year - 2004
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.30119
Subject(s) - gelatin , glutaraldehyde , rheology , dynamic mechanical analysis , materials science , chemistry , chemical engineering , composite material , chromatography , biochemistry , polymer , engineering
Abstract The definitive goal of this research is to develop protein‐based scaffolds for use in soft tissue regeneration, particularly in the field of dermal healing. The premise of this investigation was to characterize the mechanical properties of gelatin cross‐linked with microbial transglutaminase (mTGase) and to investigate the cytocompatibility of mTGase cross‐linked gelatin. Dynamic rheological analysis revealed a significant increase in the storage modulus and thermal stability of gelatin after cross‐linking with mTGase. Static, unconfined compression tests showed an increase in Young's modulus of gelatin gels after mTGase cross‐linking. A comparable increase in gel strength was observed with 0.03% mTGase and 0.25% glutaraldehyde cross‐linked gelatin gels. In vitro studies using 3T3 fibroblasts indicated cytotoxicity at a concentration of 0.05% mTGase after 72 h. However, no significant inhibition of cell proliferation was seen with cells grown on lower concentrations of mTGase cross‐linked gelatin substrates. The mechanical improvement and cytocompatibility of mTGase cross‐linked gelatin suggests mTGase has potential for use in stabilizing gelatin gels for tissue‐engineering applications. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 72B: 37–42, 2005